Installing from source

Essentials

If you don't have apt-get, Homebrew, or conda, here's the list of requirements and URLs with installation instructions:

• Ruby: https://www.ruby-lang.org/. Required: v2.1+, recommended: v2.3+.

• Python: https://www.python.org/.

• R: http://www.r-project.org/.

• SQLite3: https://www.sqlite.org/.

• NCBI BLAST+: ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST.

• HMMer: http://hmmer.janelia.org/software. Required: v3.0+.

• Bedtools: http://bedtools.readthedocs.org/en/latest/.

• Prodigal: http://prodigal.ornl.gov.

• IDBA: http://i.cs.hku.hk/~alse/hkubrg/projects/idba. Required flavor:

  IDBA-UD.

• MCL: http://micans.org/mcl/.

• Barrnap: http://www.vicbioinformatics.com/software.barrnap.shtml.

• Scythe: https://github.com/vsbuffalo/scythe. Required: v0.991+.

• FastQC: http://www.bioinformatics.babraham.ac.uk/projects/fastqc.

• SolexaQA++: http://solexaqa.sourceforge.net. Required: v3.1.3+.

• FastANI (optional): https://github.com/ParBLiSS/FastANI. Required: v1.1+.

• Diamond (optional): http://ab.inf.uni-tuebingen.de/software/diamond. Required: v0.9.20+.

Diamond is optional but strongly recommended. Indexing can be performed much faster with Diamond, searching of some databases depend on it, and it's required by the MyTaxa utils. FastANI is also recommended, since searching some databases depend on it.

R packages

The full list of R packages is automatically installed by MiGA. However, we will install one package here to make sure everything is properly initialized.

Rscript -e "install.packages('ape', repos = 'http://cran.rstudio.com/')"

MyTaxa utils

If you want to activate the MyTaxa and MyTaxa Scan steps, follow the instructions to install the MyTaxa Utils.